Microtubule-bound MKLP2 motor domain in the presence of AMPPNP
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
eLife (2017) 6 [ Pubmed: 28826477 DOI: doi:10.7554/eLife.27793 ]
- Tubulin alpha chain (50 kDa, Protein from Bovine)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Complex of 13pf microtubule with bound mklp2 motor domain in the presence of amppnp (Complex from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha and beta chains (Complex)
- Tubulin beta-2b chain (49 kDa, Protein from Bovine)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Kinesin-like protein kif20a (Complex from Bos taurus)
- Kinesin-like protein kif20a (56 kDa, Protein from Mus musculus)
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Bell KM, Cha HK, Sindelar CV, Cochran JC
J Biol Chem (2017) 292 pp. 14680-14694 [ DOI: doi:10.1074/jbc.M117.797662 Pubmed: 28701465 ]
Resolution: 9.3 Å
EM Method: Single-particle
Fitted PDBs: 5m5i, 5m5l, 5m5m, 5m5n, 5m5o
Q-score: 0.0885
Britto M, Goulet A, Rizvi S, von Loeffelholz O, Moores CA, Cross RA
PNAS (2016) 113 pp. E7483-E7489 [ DOI: doi:10.1073/pnas.1611581113 Pubmed: 27834216 ]
- Kinesin-like protein cut7 (40 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-2b chain (49 kDa, Protein from Bovine)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tubulin alpha-1d chain (50 kDa, Protein from Bovine)
- Microtubule-bound s.pombe kinesin-5 motor domain in the amppnp state (140 kDa, Complex)
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Rigor myosin X co-complexed with an actin filament
Resolution: 9.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 5kg8
Q-score: 0.212
Ropars V, Yang Z, Isabet T, Blanc F, Zhou K, Lin T, Liu X, Hissier P, Samazan F, Amigues B, Yang ED, Park H, Pylypenko O, Cecchini M, Sindelar CV, Sweeney HL, Houdusse A
Nat Commun (2016) 7 pp. 12456-12456 [ DOI: doi:10.1038/ncomms12456 Pubmed: 27580874 ]
Electron cryo-microscopy of microtubule-bound human kinesin-5 motor domain in the ADP.AlFx state.
Resolution: 9.2 Å
EM Method: Single-particle
Fitted PDBs: 4ck6, 4ck7
Q-score: 0.106
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ Pubmed: 24449904 DOI: doi:10.1073/pnas.1319848111 ]
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]
- Alpha tubulin (Protein from Bos taurus)
- 13-protofilament microtubule-bound human kinesin-5 motor domain with adp.alfx bound. a gold cluster is covalently attached to the neck linker (v365c). (Sample)
- Beta tubulin (Protein from Bos taurus)
- Kinesin-5 motor domain (Protein from Homo sapiens)
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]
cryo-electron microscopy of microtubule-bound human kinesin-5 motor domain in the ADP state
Resolution: 10.0 Å
EM Method: Single-particle
Fitted PDBs: 4ck5
Q-score: 0.096
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ Pubmed: 24449904 DOI: doi:10.1073/pnas.1319848111 ]
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]